An alternate high yielding inexpensive procedure for the purification of concanavalin A
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چکیده
Concanavalin A (Con A) purification method was developed by using calcium alginate cellulose beads containing cobalt (II) ions. The yield of Con A obtained by calcium alginate cellulose beads containing cobalt (II) ions was 6.8% as compared to 3.65% yield obtained by Sephadex G-50 method. Purified Con A agglutinated trypsin-treated rabbit red blood cells. FTIR spectra showed the presence of β sheet structure of purified Con A. AFM and SEM images confirmed the morphology of purified Con A. Con A precipitin reaction with dextran and ovalbumin was investigated by the UV-visible spectrophotometer. The turbidity measured for the binding of dextran and ovalbumin was decreased in the presence of these salts. These findings lead us to conclusion that our purification method by using calcium alginate cellulose beads containing cobalt (II) ions is better than the classical method of sephadex in terms of protein yield.
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